Table 4.

DNA binding by yCAF1.

DOI: http://dx.doi.org/10.7554/eLife.23474.011

Protein sample	DNA substrate	KD [μM] *	Hill coefficient
yCAF1	147 bp	2.1 ± 0.1	2.0 ± 0.4
	84 bp	2.3 ± 0.3	2.2 ± 0.3
	42 bp	2.5 ± 0.5	2.2 ± 0.4
	17 bp	5.1 ± 1.0	1.3 ± 0.2
yCAF1U	147 bp	4.1 ± 1.9	4.3 ± 5.8
yCAF1V	147 bp	>10	N.D.
yCAF1X	147 bp	5.7 ± 1. 7	2.9 ± 0.6
yCAF1T	147 bp	>10	N.D.

*Values determined from experiments using the 147, 84, 42 or 17 bp DNA fragment. Errors, where reported, correspond to the SEM value of three technical replicates.