Table 6.
Summary of SEC-MALLS data. Column labeling: Ve (elution Volume); MMSLS (Molar masses determined by SEC-MALLS); MMth (theoretical molar mass calculated). When there are more than two proteins in the injected sample, ‘+' indicates the mixing order. In sample 4, a five-fold molar excess of a preformed yAsf1-H3-H4 complex was incubated with yCAF1 before injection. In sample 9, a five-fold molar excess of a preformed MCM2-H3-H4 complex was incubated with yCAF1. The errors reported are the residual standard deviations of the observed data from the fitted values calculated using Astra.
| Peak 1 | Peak 2 | Peak 3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample |
Ve
(ml) |
MMsls
(kDa) |
MMth
(kDa) |
Ve
(ml) |
MMsls
(kDa) |
MMth
(kDa) |
Ve
(ml) |
MMsls
(kDa) |
MMth
(kDa) |
| yCAF1 | 9.96 | 172.1 ± 0.1 | 174.0 | – | – | – | – | – | – |
| yCAF1 + yAsf1 | 9.96 | 171.1 ± 0.6 | 174.0 | 12.91 | 38.6 ± 0.2 | 31.6 | – | – | – |
| yCAF1 + yAsf1 + H3 H4 | 10.00 | 185.3 ± 0.1 | 200.7 | 12.85 | 64.3 ± 0.2 | 58.3 | – | – | – |
| yCAF1 + yAsf1-H3-H4 1 :5 | 9.98 | 192.9 ± 0.2 | 200.7 | 12.88 | 60.1 ± 0.1 | 58.3 | – | – | – |
| yAsf1 | – | – | – | 12.96 | 36.3 ± 0.1 | 31.6 | – | – | – |
| yAsf1 + H3 H4 | – | – | – | 12.86 | 58.7 ± 0.2 | 58.3 | – | – | – |
| yCAF1 + MCM2 | 9.95 | 174.1 ± 0.7 | 174.0 | – | – | – | 14.32 | 19.1 ± 0.5 | 17.6 |
| yCAF1 + MCM2 + H3 H4 | 9.97 | 185.9 ± 0.1 | 200.7 | – | – | – | – | – | – |
| yCAF1 + MCM2-H3-H4 1 :5 | 9.98 | 190.5 ± 0.7 | 200.7 | 12.09 | 90.8 ± 0.4 | 71.0 | – | – | – |
| MCM2 | – | – | – | – | – | – | 14.32 | 18.8 ± 0.1 | 17.6 |
| MCM2 + H3 H4 | – | – | – | 12.16 | 82.6 ± 0.4 | 71.0 | – | - | – |
| yCAF1 + H3 H4 | 9.97 | 191.3 ± 0.4 | 200.7 | – | – | – | – | – | – |
| yCAF1 + yAsf1+Mmc2 + H3 H4 | 10.05 | 188.8 ± 0.9 | 200.7 | 12.79 | 67.5 ± 0.1 | 75.9 | 14.38 | 27.4 ± 0.1 | 17.6 |