Video 2. Two distinct cables of Tropomyosin Cdc8 load onto a single actin filament, related to Figure 2.

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DOI: 10.7554/eLife.23152.010

Two-color TIRF microscopy of 1.5 μM actin (Alexa-488 labeled) with 1.25 μM tropomyosin Cdc8 (Cy5-labeled). Green arrowhead indicates elongating F-actin barbed end. Purple arrowhead indicates the loading and spreading of Cdc8 cables. Scale bar, 5 μm. Time in sec.

DOI: http://dx.doi.org/10.7554/eLife.23152.010