Name	Features	Promoter/Vector	Details
ERM-APEX2	C1(1-27)-APEX2-V5-Stop	CMV/pLX304	Soybean APEX2 has four mutations relative to wild-type ascorbate peroxidase: K14D, W41F, E112K, and A134P (Lam et al., 2015). C1(1-27) indicates the first 27 amino acids from rabbit cytochrome P450 2C1 (Ahn et al., 1993): MDPVVVLGLCLSCLLLLSLW KQSYGGG V5: GKPIPNPLLGLDST This lentiviral vector carries a blasticidin resistance marker.
APEX2-OMM	Flag-APEX2-MAVS(510-540)-Stop	CMV/pLX304	Flag: DYKDDDDK MAVS(510-540) (Seth et al., 2005): RPSPGALWLQVAVTGVLVVT LLVVLYRRRLH This lentiviral vector carries a blasticidin resistance marker.
APEX2-NES	Flag-APEX2-NES-Stop	CMV/pLX304	NES: LQLPPLERLTLD This lentiviral vector carries a blasticidin resistance marker.
SYNJ2BP-V5	SYNJ2BP-linker-V5-Stop	CMV/pLX_TRC208	The SYNJ2BP gene was originally obtained as SYNJ2BP-linker-V5 in pLX304 from the Broad Institute. We made a CRISPR-resistant version and moved the construct to this pLX_TRC208. Linker: CPTFLYKVV This lentiviral vector carries a hygromycin resistance marker.
mito-tagBFP	mito-tagBFP-Stop	CMV/pcDNA3	The tagBFP (blue fluorescent protein) gene was cloned from pHR-SFFV-dCas9-BFP, a gift from Stanley Qi and Jonathan Weissman (Addgene plasmid # 46910) (Gilbert et al., 2013). Mitochondrial matrix targeting sequence (‘mito’): MLATRVFSLVGKRAISTSVCVR
V5-GFP-RRBP1	Calreticulin signal peptide-V5-GFP-RRBP1-Stop	CMV/pcDNA4 HisMax	GFP (green fluorescent protein)-RRBP1 in pcDNA4 HisMax was a gift from Alexander F. Palazzo (University of Toronto) (Cui et al., 2012). The RRBP1 gene is the full-length version with 54 decapeptide repeats and the three amino acids after the decapeptide repeat region.
V5-APEX2-NLS	V5-APEX2-NLS-Stop	CMV/pcDNA3	NLS: SRADPKKKRKVDPKKKRKVD PKKKRKV
Flag-APEX2-NLS	Flag-APEX2-NLS-Stop	CMV/pcDNA3
Flag-APEX2-CAAX	Flag-APEX2-CAAX-Stop	CMV/pcDNA3	CAAX: RSKLNPPDESGPGCMSCKCV LS