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. Author manuscript; available in PMC: 2017 Apr 25.
Published in final edited form as: J Med Chem. 2009 Jan 8;52(1):74–86. doi: 10.1021/jm800937p

Figure 1.

Figure 1

Inhibition of Pim-1 in a cell-free kinase assay. His-tagged 4E-BP1 protein was incubated with 0.1 µg Pim-1 protein kinase for 1 h at 30 °C along with [γ-32P]ATP and DMSO (solvent control, lane 1), 1.5 µM 4a (lane 2), 3 µM 4a (lane 3), 2.5 µM F7 (lane 4), 5 µM F7 (lane 5), 2.5 µM H4 (lane 6), 5 µM H4 (lane 7), or 0.5 µM (S)-6 (lane 8), respectively, as described in the Experimental Section. As a negative control, the sample in lane 9 lacked 4E-BP1. Radiolabeled 4E-BP1 was visualized by gel electrophoresis and autoradiography.