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. Author manuscript; available in PMC: 2017 Apr 25.
Published in final edited form as: J Med Chem. 2009 Jan 8;52(1):74–86. doi: 10.1021/jm800937p

Figure 2.

Figure 2

Inhibition of Pim-1 autophosphorylation in intact cells. cDNA-encoding Flag-Pim-1 was transfected into HEK-293T cells and cells were labeled with 32PO4 for 3 h. The following compounds (0.5 µM) were added and the incubations were continued for an additional 1 h: (S)-6 (lane 1), H4 (lane 2), 4a (lane 3), F7 (lane 4), or DMSO (solvent control, lane 5). Pim-1 was immunoprecipitated with Flag beads and 1/10 of the beads were analyzed by Western blotting (input), while the remaining material was analyzed by SDS-PAGE and autoradiography to visualize the autophosphorylation level of Pim-1 as described in the Experimental Section.

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