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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Alcohol Clin Exp Res. 2017 Mar 30;41(5):911–928. doi: 10.1111/acer.13362

Figure 5. Bioinformatic analysis of correlation of Col6a3, Klf12 or Ryr3 expression with ethanol behaviors in C57BL/6J × DBA/2J recombinant inbred (BXD) mouse lines.

Figure 5

The GeneNetwork (GN) web-based analysis platform was used to identify correlations between between basal expression levels of Col6a3, Klf12 or Ryr3 and ethanol behaviors. Panels A-D display Spearman ranked order correlations between microarray gene expression (x-axis) and behavioral phenotypes (y-axis). Col6a3 (probeset 1424131_at) in GN113 whole brain expression dataset showed significant positive correlation with total handling induced convulsion (HIC) score (sum of baseline subtracted HIC at 4, 6 and 7 hrs) after 4 g/kg intraperitoneal (IP) ethanol in males (GN record 11382; Panel A) and negative correlation with ethanol 2-bottle choice voluntary consumption (GN record 10479; Panel B). Klf12 basal expression in prefrontal cortex (GN135, probeset 1455521_at) was significantly positively correlated with locomotor activity 0–5 minutes after 2.25 g/kg IP ethanol (GN record 11708; Panel C) and in nucleus accumbens (GN156, probeset 1439827_s_at) was positively correlated with ethanol acute functional tolerance (GN record 10348; Panel D).