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. Author manuscript; available in PMC: 2017 Sep 2.
Published in final edited form as: Nat Neurosci. 2017 Mar 2;20(5):753–759. doi: 10.1038/nn.4534

Figure 1. Human iPSCs are differentiated into microglia-like cells.

Figure 1

(a) Schematic representation of the process used to differentiate human iPSCs into microglia-like cells. (b–j) Phenotypic staining at various steps of the differentiation process. Human iPSCs, Stage 1 hematopoietic progenitor-like cells, and Stage 2 hiPS-microglia immunostained with antibodies to Nanog and Tra-1-81 (b,e,h), CD34 and CD43 (c,f,i), CD11b and iba 1 (d,g,j), respectively. DAPI was used to visualize nuclei. The figure is representative of three independent experiments done in replicates (n=6). Scale bars, 50 μm.