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. Author manuscript; available in PMC: 2017 Sep 2.
Published in final edited form as: Nat Neurosci. 2017 Mar 2;20(5):753–759. doi: 10.1038/nn.4534

Figure 3. hiPSC-microglia demonstrate functional abilities consistent with human microglia.

Figure 3

Confocal imaging of hiPSC-MG reveals a noticeable increase in phagocytosis of pHrodo™ Red E. coli BioParticles (red fluorescence) without treatment with phorbol myristrate acetate (-PMA), however upon stimulation with PMA, they show production of reactive oxygen species (green fluorescence) starting at t=5 min. Hoechst 3342 (blue fluorescence) was used to visualize nuclei. The figure is representative of three independent experiments carried out in replicates (n=6). Scale bars, 50 μm.