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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Alcohol Clin Exp Res. 2017 Apr 5;41(5):895–910. doi: 10.1111/acer.13377

Fig. 7.

Fig. 7

ULK1 knockdown attenuates EtOH-induced increase in BECN1 and PIK3C3 phosphorylation. (A) C2C12 myoblasts were transfected with scrambled siRNA or ULK1 siRNA (0.4 μg) for 48 h. Cells were collected thereafter and analyzed by Western blotting using ULK1 antibody. (B) Myoblasts were incubated with EtOH for 15 min and equal amount of cell lysates from scrambled control or ULK1 knockdown cells were analyzed via Western blotting using antibodies against p-BECN1 at S14 (B–C), S93 (B & D) and p-PIK3C3 S164 (B & E). Each bar represents the mean ± SEM of 3 independent experiments consisting of 4 replicate samples per experiment. Results were normalized to total protein and expressed as a percentage of scrambled control levels. Groups with different letters are significantly different from one another (P< 0.05). Group with the same letters are not significantly different. *P< 0.05 versus scrambled control values.