Figure 3.
High Speed Image Analysis Reveals Dilated Heart Tube in ECM KD Flies and Prevention of Age-Related Tube Restriction (A) Still ventral images of fly heart from 120 fps video capture used to create motion-mode kymographs. Lines depict where measurements for dimensions (diastole, red, and systole, green) are taken in segment a2. (B) Diastolic heart diameters were quantified at 1 and 5 weeks for indicated genotypes. (C) Systolic heart diameters were quantified at 1 and 5 weeks for indicated genotypes. (D) Fractional Shortening were quantified for indicated genotypes and ages. All data represented as mean ± s.e.m. n>20. All data were analyzed via 2-way-ANOVA with post hoc Bonferroni test. Non-parametric student’s two-tailed t-test was used to assess significance within each genotype with age. *p< 0.05, **p< 0.01, ***p<0.001.
