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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Curr Genet. 2016 Oct 25;63(3):519–530. doi: 10.1007/s00294-016-0659-2

Fig. 6.

Fig. 6

Independent confirmation that PRE3 is essential in C. neoformans by targeted gene replacement into the diploid AI187 strain, sporulation and analysis of progeny. A. Diagram of the wild type and gene replacement alleles, with the positions of primers marked with arrows. B. PCR confirmation of correct replacement of PRE3. Faint bands in the wild type are non-specific amplification. The sizes are the 1 kb+ ladder (Invitrogen). C. Microcolony arrested at a four-cell stage after basidiospore germination on YP glucose agar. D. Segregation data of colonies after sporulation of the heterozygous pre3/PRE3 strain; the pre3/PRE3 strain is in the bottom right corner. Mating types are grey for MATa and white for MATα.