Table 2.
Summary of insertional mutants and the roles of the essential genes.
| Strain name |
Gene affected |
H99 gene designation |
Position in gene |
Protein encoded by gene | References |
|---|---|---|---|---|---|
| 1A12 | NOC3 | CNAG_04298 | Promoter | Nucleolar factor required for rRNA maturation and DNA replication. |
(Milkereit et al. 2001; Zhang et al. 2002) |
| 3A11 | PRE3 | CNAG_03816 | 5´ UTR | Proteosome subunit. | (Enenkel et al. 1994) |
| 3F5 | MRPL37 | CNAG_04664 | Promoter | Protein in the mitochondrial ribosome large subunit |
(Hanlon et al. 2004; Merz and Westermann 2009) |
| 4C9 | IDI1 | CNAG_00265 | 5´ UTR | Isopentenyl diphosphate:dimethylallyl diphosphate isomerase, required for isoprenoid biosynthesis. |
(Mayer et al. 1992) |
| 8A11 | RPL17 | CNAG_06447 | Exon 2 | Ribosomal protein L17 of the 60S subunit. |
(Gamalinda et al. 2013) |
| 8D9 | NOP56 | CNAG_02209 | 5´ UTR | Nucleolar factor required for rRNA maturation. |
(Gautier et al. 1997) |