Fig. 4.

Mycobacteria-specific cytokine responses in cryopreserved PBMC stimulated overnight with PPD or 85A peptide pools. Comparable levels of PPD (A) and 85A-specific (B) IFNγ, TNFα and IL2 were detected with no significant differences between the two vaccination groups at any time-point (Mann Whitney U test). Significant cytokine production was detected at all time-points compared to baseline (p < 0.05, Wilcoxon matched pairs test). Polyfunctional PPD and 85A-specific CD4+ T cells (C) producing multiple cytokines were detected following vaccination. The patterns of cytokine production are comparable between both groups. Median values are shown. (D) Shows the expression of CCR4, CCR6, CCR7 and CXCR3 on CD4+ cells in unstimulated cryopreserved PBMC. Individual values for subjects are shown with horizontal bars representing median values.