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. 2017 Apr 26;11:62. doi: 10.3389/fnbeh.2017.00062

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Copyright © 2017 Han, Jiang, Ding, Liu, Xiao and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Open-field test: The immobilization (IM)-stressed rats showed a greater percentage of distance in the border zone compared with control rats. (B) Elevated plus maze: IM-stressed rats showed shorter distance, less time and less entry number in the open arm compared with control rats. (C) Conditioning test: The percentage of time spent freezing in contextual and auditory cue fear conditioning (post-CS) were significantly higher in the IM rats than in the control rats (*P < 0.05 vs. the control group). No significant difference was observed in baseline and pre-CS of the auditory cue fear conditioning between control and IM rats. (D) sensibility test to the foot-shock, no significant difference in minimum current strength which induced notice, flinch and vocalize was found between control and IM rats.