Fig. 3.
Optimization for AAV-GFP and CatB in vitro. a Immunoblots show expression of GFP and CatB in a dose-dependent manner in NPC-derived neurons transduced with AAVs at 1 × 107–9 vg/10,000 cells/well. b Overexpression of CatB in NPC-derived neurons has no effect on cell viability as compared to control (Con) or GFP group. c Hippocampal frozen sections were immunostained for HA to identify exogenous CatB expression. HA-immunostaining and GFP fluorescent images in the AAV-GFP or CatB-injected hippocampus were shown. Scale bar =100 μm (50 μm in high magnified images). d Hippocampal frozen sections were immunostained for GFAP (astrocyte) or Iba1 (microglia). Scale bar =200 μm. e Quantification of GFAP-positive cells in the hippocampus. f Quantification of Iba-positive cells in the hippocampus