Figure 5.

SypA Proteins from Other Vibrios are Susceptible to Phosphorylation by V. fischeri SypE. Development of colony morphology over time of sypG (pCLD56)-overexpressing derivatives of ΔsypA (sypE+) strains that contain (A) native sypA (KV5479), (B) sypA-S56A (expressing a mutant SypA that cannot be phosphorylated by SypE; KV5481), (C) rbdA (KV7309), or (D) sypA_vp (KV7313). Cultures were spotted onto LBS plates containing tet, and the morphologies of the resulting colonies were assessed at the indicated times. Representative images are shown. At 72 h, the colonies were disturbed with a toothpick to assess colony cohesiveness.