Fig. 4.

Transcriptome profiles of HLCs and validation of ANGPTL3.

(A) The overlap of genes expressed in TD-1 and control-1 HLCs was evaluated by Pearson Correlation Coefficient. (B) Heat map of pluripotency and hepatic markers between groups. (C) Heat map of differentially expressed lipid-related genes according to Gene Ontology lipid terms. (D) Expression of ANGPTL3 measured by RT-PCR. (E) Culture medium ANGPTL3 protein concentrations determined by ELISA. (F) Plasma ANGPTL3 levels were measured by ELISA. Values are shown as mean ± SEM. n = 9 for RT-PCR analysis. n = 3 for culture medium ELISA. n = 4–19 for plasma ANGPTL3 ELISA. * denotes p < 0.05.