Fig. 3.

Pharmacologic ascorbic acid induces mitochondria-mediated apoptosis in MM cells. (A) Transmission electron microscopy of OCI-MY5 WT cells treated with or without PAA (4 mM). After 1 h incubation, PAA was washed away and cells were fixed for TEM after 60 min and 120 min. Second row represents different field in the section. Red boxes represent zooming image of mitochondria in OCI-MY5 WT cells (left) and OCI-MY5 WT cells treated with PA (right). (B) OCI-MY5 WT cells were treated with or without PAA. After 1 h, PAA was washed away and cells were lysed at the specified times and RIP1, RIP3, Caspase 3, Caspase 8, Caspase 9, and β-actin levels were examined by western blots.