Fig. 6.

Maternal vitamin A increases PDGFRα⁺ population in adipose tissue. (a–d) For MVA weanling offspring. The population of PDGFRα⁺ cells in iWAT was analyzed by flow cytometry (a) (numbers in the quadrant indicate the percentages of cells) and IHC using anti-PDGFRα antibody (b). DiI labeled iWAT was further stained using anti-PDGFRα antibody (c). Brown adipose protein contents in iWAT-derived SVCs after 6 days of brown adipogenesis were analyzed (d). (e–f) PDGFRα⁺ cells in PDGFRα tracking mice were labeled by GFP in presence of tamoxifen (e). PDGFRα⁺ cells-derived adipocytes in iWAT after 1 week of cold stimulus were observed under confocal microscope (f).