TABLE 4.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Reference(s) or source
V. cholerae
N16961	O1, El Tor strain, Bangladesh, clinical, 1975, VPI-1, VPI-2, VSP-I, VSP-II	59
SG7	Non-O1, nonclinical, VPI negative	Lab collection
JDBIntV1	RC385, intV1 deletion	This study
MRCIntV2	NRT36S, intV2 deletion	This study
VPI-6 CRISPR strains
RC385	O135, Chesapeake Bay, USA, plankton, 1998	14, 60
RC586	O133, Chesapeake Bay, USA, water, 1999	15, 16
TM11079-80	O1, El Tor, Brazil, sewage, 1980	14, 16
HC-36A1	−, Haiti, clinical, 2010	43
VPI-3 T3SS strains
NRT36S	O31, Japan, clinical	34
AM-19926	O39, Bangladesh, clinical, 2001	16
TMA-21	−, Brazil, sewage, 1980	16
VC12129	O1, El Tor, Australia, water, 1985	16
EM1676A	O1, Bangladesh, water, 2011
NHCC008d	−, Bangladesh, clinical, 2010	43
VC35	−, Malaysia, clinical, 2004	43
HE-25	−, Haiti, water, 2010
P-18785	−, Russia, clinical, 2005
CP1115	O75, Florida, USA, clinical, 2010
1587	O12, Peru, clinical, 1994	16
623-39	−, Bangladesh, water, 2002	16
E. coli
DH5αλpir		Lab collection
β2155λpir	Donor for bacterial conjugation, DAP auxotroph	Lab collection
Plasmids
pJET1.2	Cloning vector, Ampr
pJET1.2ΔintV1	pJET1.2 harboring truncated intV1 gene	Fermentas
pJET1.2ΔintV2	pJET1.2 harboring truncated intV2 gene	Fermentas
pDS132	Suicide vector, SacB, Cmr	Lab collection
pDS132ΔintV1	pDS132 harboring truncated intV1 gene	This study
pDS132ΔintV2	pDS132 harboring truncated intV2 gene	This study