FIG 4.

Identification of an MdbA-VKOR intermediate complex. (A and B) Protein samples obtained from the membrane fractions of A. oris strains were boiled in SDS-containing sample buffer in the presence (A) or absence (B) of 10% β-mercaptoethanol (BME) and subjected to SDS-PAGE, followed by immunoblotting with anti-VKOR or anti-MdbA Ab. Arrowheads indicate high-molecular-mass MdbA-VKOR complexes. (C) Clear lysates obtained from membrane fractions of the ΔVKOR mutant strains expressing wild-type VKOR and VKOR variants were incubated with anti-HA Ab–agarose beads, followed by immunoblotting with anti-VKOR or anti-MdbA Ab. The MdbA-VKOR complex (arrowheads) and molecular mass markers are indicated.