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. 2017 Apr 25;199(10):e00889-16. doi: 10.1128/JB.00889-16

FIG 4.

FIG 4

FeEnt transport by A. baumannii. (A) Growth of A. baumannii 17978 (circles) and its ΔfepA derivative (squares) in MOPS minimal medium without (open symbols) or with (gray symbols) 100 μM apoFcA (means from 3 experiments with standard deviations of the means as error bars) shows no growth defect from the ΔfepA mutation. (B) A. baumannii 17978 (●) and its ΔfepA derivative (■) were grown in LB, subcultured (1%) into MOPS minimal medium plus 100 μM apoFcA, and subcultured (1%) into MOPS minimal medium plus or minus 1 μM FeEnt (means and standard deviations from three experiments). The ΔfepA strain shows roughly half the growth of its parent. (C and D) Siderophore nutrition assays with A. baumannii 17978 and its ΔfepA mutant (C) or 17978/pWH1266 (++/p), ΔfepA/pWH1266 (ΔfepA/p), ΔfepA/pWH1266AbafepA+ (ΔfepA/pAbafepA+), and ΔfepA/p2682proAbafepA+ (ΔfepA/pproAbafepA+) (D). The bacteria were plated in top agar with 100 μM apoFcA, and discs containing FeEnt or Fc (10 μl of 50 μM) were placed on the agar surface. Both panels C and D are representative images from three separate experiments. (E) Accumulation of [59Fe]Ent over 45 min, with each data point performed in triplicate, by 17978/pWH1266 (●), ΔfepA/pWH1266 (■), and ΔfepA/p WH1266AbafepA+ (▲). The graph depicts the means and standard deviations of the means from two experiments. (F) Transport of 59Fe[Ent] by A. baumannii 17978 (●) or its ΔfepA mutant (■). The graph depicts the means and standard deviations of the means from triplicate measurements in two separate experiments.