FIG 4 .

HCV inhibition of p53 activation is not mediated by HCV polyprotein expression. (A) Immunoblots of p53, HCV core, and HCV NS5B in UHCV-11 cells maintained in the presence of 1 μg/ml tetracycline (+) or absence of tetracycline (−) and treated with 50 μM etoposide (ETOP) (+) or without ETOP (−) for 6 h. β-Actin was used as a loading control. (B) Flow cytometric analysis of p53 and HCV core protein expression in UHCV-11 cells treated as described for panel A. (C) Flow cytometric analysis of p21 and HCV core protein expression in UHCV-11 cells treated as described for panel A. Quadrants are based on staining with isotype control antibodies. The frequency of events in each quadrant is represented as the percentage of total gated events.