TABLE 3.
Flock-level comparison of clinical diagnosis and aprV2/B2 qPCR using 758 lesion swabs collected from 40 Australian sheep flocksa
| Clinical diagnosisb | No. of flocks with a laboratory diagnosis (aprV2/B2 qPCR) of:c |
||
|---|---|---|---|
| Benign | Virulent | Total | |
| Benign | 5 | 11 | 16 |
| Virulent | 0 | 24 | 24 |
| Total | 5 | 35 | 40 |
McNemar's χ2 = 11.0, P = 0.0009; kappa statistic = 0.353 (95% CI, 0.105–0.601), DSe = 100% (95% CI, 87.5–100%), DSp = 31.3% (95% CI, 11.0–58.6%).
Clinical diagnoses are given in Table 1.
qPCR according to reference 21. Benign, swabs tested negative for the aprV2 allele; virulent, ≥1 swab tested positive for the aprV2 allele.