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Distribution of urine specimens according to ZIKV reference PCR, Altona PCR, and NS5 gene PCR results. ZIKV, Zika virus; reference PCR, 2-step dual-target real-time reverse transcription-PCR, designed by the U.S. Centers for Disease Control and Prevention, targeting the Zika virus envelope (E) and premembrane (prM) genes; NS5, nonstructural protein 5; NT, not tested.
