FIGURE 2.

RNAi-mediated knockdown of p300 reduces acetylation of various Atg constructs. HeLa cells were assessed after control or p300-specific knockdown for the level of Atg acetylation. Various Myc-tagged Atg constructs were employed including the following: A, Atg5; B, Atg7; C, Atg8; and D, Atg12. Level of acetylation was determined by immunoprecipitation (IP) of transfected protein cell lysate (2 mg) with an acetyl-lysine antibody followed by Western blotting (WB) employing the Myc epitope. In each case, p300 knockdown reduced the level of Atg acetylation without altering the level of Atg expression.