FIGURE 3.

Increased expression of p300 augments acetylation of various essential autophagy components. HeLa cells were transiently transfected with the indicated construct encoding for a Myc-tagged Atg family member along with either a p300 expression vector or the empty vector alone (-). Levels of Atg5 (A), Atg7 (B), Atg8 (C), or Atg12 (D) acetylation were determined by immunoprecipitation (IP) of protein lysate (2 mg) using an antibody recognizing internal acetyl-lysine residues followed by Western blotting (WB) employing the Myc epitope. Expression levels of the various transfected myc-Atg constructs were assessed by Western blotting of the protein input (40 μg).