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. Author manuscript; available in PMC: 2017 Apr 26.
Published in final edited form as: Adv Healthc Mater. 2016 May 24;5(13):1656–1666. doi: 10.1002/adhm.201600136

Figure 6.

Figure 6

ECM accumulation and compressive modulus of hMSC-seeded collagen foams after 6 weeks of culture. A) Sulfated glycosaminoglycan (sGAG) content of tissue deposited by hMSCs in collagen foams after 6 weeks in culture. B) Hydroxyproline content of tissue deposited by hMSCs in collagen foams after 6 weeks in culture as an estimation of total collagen content. C) Dynamic mechanical analysis (DMA) used to determine the elastic modulus of compression of hMSC-seeded collagen foams compressed to 10% strain at 0.5% strain min−1 and 1 Hz after 6 weeks in culture. Values represent means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 for each donor; 3 different bone marrow-derived hMSC donors). D) Representative histological and immunohistochemical examination of hMSC-seeded collagen foams after 6 weeks in culture. Collagen foams are stained with hematoxylin and eosin (H&E), alcian blue for sGAG, and by immunohistochemistry for collagen type I, collagen type II, collagen type X, and aggrecan, from top to bottom. Scale bars are 25 μm.