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. 2017 Apr 26;7:46668. doi: 10.1038/srep46668

Figure 5. Influence of autophagy inhibition on toxicity associated with mild MPP+ exposure.

Figure 5

(a) SH-SY5Y cells were transfected with siRNA specific for autophagy protein 5 (siAtg5) or scrambled control siRNA for 24 h and subsequently exposed to 10 and 200 μM MPP+ for 48 h. LC3-II and Atg5 expression levels were analysed by western blotting. (b) SH-SY5Y cells were transfected with siAtg5 or scrambled control siRNA for 24 h, followed by exposure to 10 and 200 μM MPP+ for 48 h. Cell viability was determined by a WST-1 assay. (c) SH-SY5Y cells were exposed to 10 and 200 μM MPP+ for 48 h with or without 5 mM 3-MA for the last 24 h; LC3-II expression was analysed by western blotting. (d) SH-SY5Y cells were exposed to 10 and 200 μM MPP+ for 48 h with or without 5 mM 3-MA for the last 24 h; cell viability was determined by the WST-1 assay. Data are expressed as the means ± S.D. from at least three independent experiments.