Table 2. Primer sequences for qPCR analysis.
Target | Forward Primer 5′–3′ | Reverse Primer 3′–5′ | Genebank Acc. |
---|---|---|---|
KCNK1 | CTACCTGGTGTTCGGCGCCG | CTGCGGGTGACATGCACGGT | NM_008430.2 |
KCNK2v1 | ATGCTGCATGCCTCATGCTTGCC | ACACCGTGGCTCCGATGATCA | NM_001159850.1 |
KCNK2v2 | CTGCAGTGATCACCCCCTCGC | TATTCCAAGACGGGCTGCGCG | NM_010607.3 |
KCNK3 | TGGCTCTCATCGTGTGCACCTTC | GAACACCTTGCCTCCGTCCGT | NM_010608 |
KCNK4 | GTGACCCAGCGAACTGGGCC | GCCACGCTCACTCTGCGTGT | NM_008431 |
KCNK5 | ACCCATGGCTGAGGCACCCT | AGGCCTGTCCTGGGCCAACA | NM_021542 |
KCNK6 | TCCCAGACTCAGTGCCATGCTATGG | TGTGCTCTCAGGACCGCACCTA | NM_001033525 |
KCNK7 | TTGCTGCCTGCCCATGGACG | CTCCCGCTCAGGTCCCCCAA | NM_010609 |
KCNK9 | AGCGGCAGAACGTGCGTACC | AGGTGTTCATGCGCTCGCCC | NM_001033876 |
KCNK10 | GCGAGACCCAACCACTCCGC | GGCCCGGAAGACAAGGCCAC | NM_029911 |
KCNK12 | CACGCTGCGCAACTTCAGCG | GCGCAGCCGAACAGTCCGTA | NM_199251 |
KCNK13v1 | CTCCAGAGCTCATGCGGTCCG | TGCTTCGACTTGGTGCGAGGG | NM_001164427.1 |
KCNK13v2 | GGTGCGTTTGGGAAGCGGTCC | GGTCCGGGTTCCTCGGAGCAT | NM_001164426.1 |
KCNK13v3 | ACCTGTTCCACAGAGCAGCGGTC | TCGGTGCCTCTCCAGGTGGATC | NM_146037.2 |
KCNK15 | TACCGCTTCTCCGCCGACGA | AGGCAGCGCTTGGCTGTCAG | NM_001030292.1 |
KCNK16 | AGACCAGCAGGCCCTGGAGC | ATGCCTGGGGTGGTCCTCCC | NM_029006.1 |
KCNK18 | GCTCTGCCGGAAGCAGCCTG | CCTCTCCACCTGCTGGCCCA | NM_207261.3 |
GAPDH | TGCACCACCAACTGCTTAG | GATGCAGGGATGATGTTC | NM_008084.2 |
ARBP | CGACCTGGAAGTCCAACTAC | ATCTGCTGCATCTGCTTG | NM_007475.5 |
PSMB2 | AAATGCGGAATGGATATGAAT | GAAGACAGTCAGCCAGGTT | NM_011970.4 |
Β-actin | ACCAACTGGGACGATATGGAGAAGA | CGCACGATTTCCCTCTCAGC | NM_007393.3 |
v1, v2 and v3 refer to splice variant isoforms of these genes.