Figure 6. Observed and expected trend lines between T cell functional output and effective kinetic constants, derived from the 3D FRET and SPR assays, for the set of peptides used in Fig. 1.
The dark blue lines correspond to the effective kinetic constants 
, 
, and 
from the SPR assay. The cyan lines are the predicted trend lines for the correlation between log(1/EC50) and the logarithm of the effective parameters 
, 
, and 
from the 3D FRET assay, as inferred using the results in Case 5. Dashed vertical lines are drawn to help visualize the asymptotic limits of 
and 
. Their particular positions, as well as that of cyan lines, are for illustrative purposes.