Table 1.
Summary of the Reaction Profiles of the Three Cysteine Modifiers the Full Length Protein, βαMT, and the two Isolated Fragments Under Native and Denaturing Conditions
| Protein construct | Modifier | Native reaction profile | Denatured reaction profile |
|---|---|---|---|
| βαMT1a | NEM | Cooperative | Non‐cooperative, stochastic |
| Bq31 | Cooperative | Non‐cooperative, stochastic | |
| IAM | Non‐cooperative, stochastic, wide distribution | Non‐cooperative, stochastic, narrow distribution | |
| αMT1a | NEM | Cooperative | Non‐cooperative, stochastic |
| Bq25 | Cooperative | Non‐cooperative, stochastic | |
| IAM | Non‐cooperative, stochastic | Non‐cooperative, stochastic | |
| βMT1a | NEM | Cooperative | Non‐cooperative, stochastic |
| Bq | Cooperative | Non‐cooperative, stochastic | |
| IAM | Non‐cooperative, stochastic | Non‐cooperative, stochastic |