. 2017 Apr 26;10:203. doi: 10.1186/s13071-017-2133-5

Table 3.

Sequences of primers based on the cytochrome b (cytb) gene that were used to identify the food sources of anopheline mosquitos [24]

Food source tested	Primer sequence	Product size (bp)
Domestic cat	TTCTCAGGATATACCCTTGACA	180
Domestic cat	GAAAGAGCCCATTGAGGAAATC	180
Human	TTCGGCGCATGAGCTGGAGTCC	228
Human	GTRTARTAGGGRTGRAATC	228
Domestic dog	GAACTAGGTCAGCCCGGTACTT	153
Domestic dog	CGGAGCACCAATTATTAACGGC	153
Birds	GACTGTGACAAAATCCCNTTCCA	508
Birds	GTCTTCATCTYHGGYTTACAAGAC	508
Reaction	94 °C for 2 min; 35 cycles of 94 °C for 30 s, 54–70 °C^a for 30 s, 72 °C for 30 s; and a final extension step at 72 °C for 20 min

^aDepending on the annealing temperature of each oligonucleotide (see Chang et al. [24])