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. 2017 Apr 26;17:49. doi: 10.1186/s12935-017-0419-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Digitized images of γH2AX and pATM foci. After exposure to 2 Gy ¹²C⁶⁺ and incubation 0.5 h for γH2AX and 4 h for pATM, cells were grown and irradiated on cover slips. DNA was stained with DAPI and γH2AX and pATM was detected using an Alexa 488-conjugated secondary antibody after staining using anti-phospho-histone H2AX (Ser-139) and anti-phospho-ATM (ser1981) mAb. a Hela-γH2AX; b Hela-pATM; c HepG2-γH2AX; d HepG2- pATM; e MEC-1-γH2AX; f MEC-1-pATM. Scale bar 15 μm