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. 2017 Apr 26;17:83. doi: 10.1186/s12870-017-1031-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — At1g59480 produces an mRNA encoding a non-functional protein. a Schematic of the At1g59480 predicted gene model (top) and experimentally determined cDNA (bottom). Exons and introns are represented as thick and thin lines, respectively. Intron 1 sequences in experimental cDNA are underlined in red and location of the predicted premature stop codon is denoted by a green asterisk. b Sequence alignment of At1g59480 genomic DNA (At1g59480_gDNA), experimentally determined cDNA (actual_cDNA), and predicted cDNA (prediction_cDNA) showing presence of DNA in the experimentally derived sequence corresponding to intron 1 underlined in red, in-frame stop codon underlined in green