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. 2017 Apr 26;17:83. doi: 10.1186/s12870-017-1031-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — A subset of Arabidopsis FRKs shares biochemical cofactor requirements. Scatterplots comparing enzyme activity of FRK1 (blue), FRK2 (red), and FRK3 (green) in various conditions normalized to that of their respective control experiment. Control experiments were carried out in a complete mixture as compared to mixtures where inorganic phosphate was omitted (−Pi), K+ was replaced with Na + to preserve ionic strength (−K+), Mg2+ was omitted and replaced with EDTA (EDTA), or ATP was replaced with either GTP (GTP) or (UTP). Statistical analysis was conducted with Dunnett’s post hoc test to compare the mean of each enzyme in each condition to the mean of the control experiment for the corresponding enzyme. *p < 0.05, ****p < 0.0001, ns = not significant. Experiments were carried out in quadruplicate, all data points are shown