Table 2.
Biochemical parameters determined for Arabidopsis FRK isozymes
| Enzyme | Km μM Fructose | Km μM ATP | Fruc substrate inh. | Vmax (μM product *min−1 *mg enzyme−1) | kcat (s−1) | Catalytic efficiency kcat/Km (ATP) |
|---|---|---|---|---|---|---|
| FRK1 | 470 +/− 77 | 52 +/− 7.8 | − | 2.3E + 05 | 14.2 | 2.7E + 05 |
| FRK2 | 370 +/− 91 | 85 +/− 8.7 | + | 1.8E + 05 | 10.3 | 1.2E + 05 |
| FRK3 | 480 +/− 99 | 52 +/− 9.8 | + | 2.1E + 05 | 14.3 | 2.7E + 05 |
| FRK4 | 230 +/− 56 | 95 +/− 7.9 | + | 1.9E + 05 | 11.1 | 1.2E + 05 |
| FRK5 | 320 +/− 89 | 180 +/− 57 | + | 7.7E + 04 | 4.8 | 2.7E + 04 |
| FRK6 | 260 +/− 81 | 160 +/− 21 | + | 2.1E + 05 | 12.8 | 8.0E + 04 |
| FRK7 | 12 +/− 8.4 | 280 +/− 94 | − | 2.6E + 04 | 1.5 | 5.4E + 03 |