Table 2.
Amounts of PduP-tagged protein isolated with BMCs
| Strain | Induction conditions | Total PduP-tagged protein in µg (% in total lysate)a | Total BMC yield (mg)b | PduP-tagged protein/BMCs (µg/mg) | ||
|---|---|---|---|---|---|---|
| Lysate | Soluble fraction | Isolated BMCs | ||||
| Ec0087 | 0.2 mM Rha | 1300 (100%) | 75 (5.8%) | 0.063 (0.0049%) | 0.062 | 1.0 |
| Ec3087 | 0.1 mM Rha + 0.5 mM IPTG | 1300 (100%) | 380 (28%) | 18 (1.4%) | 1.4 | 14 |
| Ec3087 | 0.2 mM Rha + 0.5 mM IPTG | 1700 (100%) | 170 (10%) | 8.7 (0.52%) | 0.74 | 12 |
| Ec3087 | 0.5 mM Rha + 0.5 mM IPTG | 1100 (100%) | 120 (11%) | 12 (1.1%) | 0.94 | 13 |
| Ec3090 | 0.2 mM Rha + 0.5 mM IPTG | 29,000 (100%)c | 22,000 (75%)c | 73 (0.25%)c | 0.88 | 39a |
aAmounts of PduP-tagged proteins were determined by quantitative Western blots using anti-His6 (see Additional file 1: Figure S9 for raw data)
bTotal protein content was determined by Bradford assay using BSA as a standard
cmg of PduP-mCherry was estimated based on a PduP-E standard curve, assuming anti-His6 detects an equal number of PduP-E and PduP-mCherry molecules [i.e., 1 ng of PduP-E (14.8 kDa) detected is equivalent to 2.1 ng of PduP-mCherry (31.2 kDa) detected]