Fig. 9.

CD4 and CD8 T-cell proliferation in amyloid-β peptide 1–42 (Aβ42) peptide restimulated peripheral blood mononuclear cell cultures. a Mononuclear blood cells were separated on the basis of forward scatter (FSC) and side scatter (SSC). The upper histogram shows the pattern in fresh blood, and the lower histogram shows the cell populations after 6 days in culture. Gates for the carboxyfluorescein succinimidyl ester (CFSE) assay were set on lymphocytes (E1). b T-cell proliferation was analyzed using a CFSE dilution assay. Events were gated on CD4- and CD8-positive cells, and cytometric histograms are shown for medium controls, Aβ42 peptide restimulated cultures, and proliferation after anti-CD3 antibody stimulation. All samples were run in triplicates. No T-cell proliferation in response to Aβ42 peptide restimulation was found