Fig. 5.
Effect of NACA on a Casein zymography and b Western blot, and c Relative densitometry intensities of m-calpain. a Lens from sodium selenite injected rat pups showed a decreased band intensity of m-calpain indicating decreased m-calpain activity compared to the control. However, treatment with NACA preserved the m-calpain activity; b Western blot from sodium selenite injected rat pups showed a band intensity of m-calpain compared to control; c Western blot results normalized to the levels of GAPDH gene. The graphs represent relative densitometric analysis of treated group lenses over the control group lenses. Statistical Analysis was performed by one-way analysis of variance, followed by Tukey’s multiple comparison tests. *p < 0.05 compared to the control group and #p < 0.05 compared to the Na2SeO3 group. The graph is representative of triplicate gels and the values are reported as mean ± S.E