Table 1.
Growth Rates (hr−1) | Time Lag (hr) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Metabolites | WT + chl | WT + str | WT | ChlR | StrpR | WT + chl | WT + str | WT | ChlR | StrpR |
Glucose | 0.00 | 0.00 | 0.71 | 0.21 | 1.80 | 3 | 9.5 | 2 | ||
Glucose-6-phosphate | 0.09 | 0.01 | 1.08 | 0.77 | 2.23 | 3 | 3 | 2 | 3.5 | 2 |
Glyceraldehyde 3-phosphate | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||||
Fructose 1,6-bisphosphate | 0.00 | 0.01 | 1.14 | 0.23 | 0.25 | 3 | 1.5 | 6 | 10 | |
Fumarate | 0.00 | 0.06 | 1.55 | 0.21 | 0.27 | 3 | 1.5 | 12 | 13.5 | |
Maleic acid | 0 d | 0.00 | 0.60 | 0 a | 0 c | 1 | ||||
D-Malic acid | 0.08 | 0.01 | 0.67 | 1.89 | 0 b | 2.5 | 15.5 | 1.5 | 0 | |
Succinate | 0.00 | 0.00 | 1.00 | 0 a | 0 b | 2.5 | ||||
Oxalic acid | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||||
Oxoadipic acid | 0.04 | 0.00 | 0.62 | 0 e | 0 c | 0 | 0 | |||
Malonic acid | 0.11 | 0.03 | 1.08 | 0.45 | 0.17 | 2.5 | 2.5 | 1.5 | 2 | 18 |
Pyruvate | 0.41 | 0.00 | 0.58 | 0 e | 0 b | 4 | 2.5 | |||
Citric acid | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |||||
Isocitric Acid | 0.46 | 0.00 | 1.31 | 0.32 | 0.32 | 10 | 3.5 | 3.5 | 12.5 | |
L-Lactic acid | 0.29 | 0.00 | 0.99 | 1.05 | 0.16 | 3 | 4.5 | 4.5 | 18 | |
Ketoglutaric acid | 0.15 | 0.09 | 0.19 | 0.24 | 0.04 | 0 | 0 | 3.5 | 0 | 21 |
L-Arabinose | 0.00 | 0.00 | 0.88 | 0.54 | 0.29 | 3.5 | 3.5 | 13.5 | ||
Manose 6-Phosphate | 0.18 | 0.00 | 1.08 | 0.98 | 1.68 | 3 | 2.5 | 3.5 | 2.5 | |
Ribose 5-phosphate | 0.28 | 0.00 | 0.86 | 0.33 | 0.18 | 2.5 | 2.5 | 6 | 6 | |
3-phosphoglyceric acid | 0.29 | 0.26 | 0.28 | 0.40 | 0.18 | 2 | 2 | 0 | 6 | 6 |
L-Tryptophan | 0.00 | 0.00 | 0.53 | 0.23 | 1.92 | 2 | 2 | 2 | ||
L-Alanine | 0.21 | 0.00 | 0.96 | 0.28 | 0.36 | 2.5 | 2.5 | 6 | 3.5 | |
L-Valine | 0.28 | 0.00 | 0.74 | 0.33 | 0.14 | 2 | 2 | 6 | 6 | |
L-Aspartate | 0.27 | 0.20 | 0.19 | 0.41 | 0.18 | 2.5 | 2.5 | 0 | 6 | 10 |
L-Glutamine | 0.00 | 0.00 | 0.83 | 1.15 | 0.26 | 2.5 | 3.5 | 13 | ||
L-Glutamate | 0.52 | 0.16 | 1.28 | 1.12 | 0.23 | 3 | 3 | 3 | 3 | 12.5 |
Mannitol | 0.24 | 0.00 | 1.00 | 0.98 | 0.20 | 3 | 2 | 3.5 | 8.5 | |
D-Sorbitol | 0.24 | 0.00 | 0.58 | 0.40 | 0.25 | 12.5 | 2.5 | 6 | 9.5 | |
Glycerol | 0.00 | 0.00 | 0.78 | 0.28 | 1.99 | 2.5 | 9.5 | 2.5 | ||
L-Ascorbic acid | 0.00 | 0.18 | 0.81 | 0.23 | 0.27 | 3 | 3 | 6 | 12 | |
Luria bertani | 0.00 | 0.00 | 0.47 | 0.37 | 1.69 | 3 | 3 | 1.5 | 3 | 3 |
Altered kinetic parameters represented as Growth Rates and Time lag for the three populations of C. violaceum (WT, ChlR and StrpR) on multiple micro-environment metabolites. Zero growth rates are represented in bold
at6 initial till 180 min
bt0 initial till 180 min
ct0 first 60 min
d3 to 18 h growth
egrowth after 24 h