Table 5.
FVA results showing category change in resistant strains as a function of antibiotic that involve redox cofactor balancing
| Reaction ID | Reaction formula | WT | ChlR | StrpR |
|---|---|---|---|---|
| HEX1 | atp[c] + glc-D[c] - > adp[c] + g6p[c] + h[c] | 1 | 7 | 7 |
| PYK | adp[c] + h[c] + pep[c] - > atp[c] + pyr[c] | 7 | 1 | 1 |
| AKGDH | akg[c] + coa[c] + nad[c] - > co2[c] + nadh[c] + succoa[c] | 1 | 7 | 1a |
| MDH | mal-L[c] + nad[c] < = > h[c] + nadh[c] + oaa[c] | 1 | 7 | 1a |
| FUM | fum[c] + h2o[c] < = > mal-L[c] | 1 | 7 | 1a |
| SUCOAS | atp[c] + coa[c] + succ[c] < = > adp[c] + pi[c] + succoa[c] | 4 | 7 | 4a |
| ACKr | ac[c] + atp[c] < = > actp[c] + adp[c] | 7 | 4 | 4 |
| PFL | coa[c] + pyr[c] - > accoa[c] + for[c] | 7 | 1 | 7b |
| PTAr | accoa[c] + pi[c] < = > actp[c] + coa[c] | 7 | 1 | 1 |
| GLCt2 | glc-D[e] + h[e] - > h[c] + glc-D[c] | 1 | 7 | 7 |
| ACt2r | ac[e] + h[e] < = > ac[c] + h[c] | 7 | 4 | 4 |
| FORti | for[c] - > for[e] | 7 | 1 | 7b |
| EX_ac(e) | ac[e] < => | 7 | 1 | 1 |
| EX_for(e) | for[e] < => | 7 | 1 | 7b |
Increased TCA cycle/Oxidation Phosphorylation in the StrpR population and increased overflow metabolism population diverting from TCA cycle in the ChlR population. Of use here are Category definitions – 1 and 4 representing forced and fixed flux in either direction. 7 defined by negligible variable flux
aStrpR/WT flux fold is 0.52
bStrpR/WT flux fold is 0.32