Figure 3.

Primary human PTEC produce more exosomes under disease culture conditions but with comparable morphology. (a) Exosome numbers produced by equivalent cell culture volumes under normal and disease (inflammation and hypoxia) culture conditions. All exosomes purified using the Kit methodology. Results represent mean ± SEM of six individual PTEC donor experiments. *p < 0.05 by one-way analysis of variance with Bonferroni’s multiple-comparison test. (b) Equivalent size distribution of exosomes derived from primary human PTEC under normal and disease conditions; analysed with TRPS (qNano, Izon Science Ltd. Christchurch, New Zealand) using a NP100 nanopore at a 45 mm stretch. All exosomes purified using the Kit methodology. Results represent mean ± SEM of six individual PTEC donor experiments. (c) Electron microscopy images of exosomes purified from PTEC cultured under normal and disease conditions. Scale bars = 100 nm. All exosomes purified using the Kit methodology.