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Primary human PTEC cultured under disease conditions produce greater numbers of exosomes – defined by CD9/CD63/CD81 content. Western blot of whole exosome lysates (5 µg total protein) following purification of exosomes from equivalent cell culture volumes under normal and disease (inflammation and hypoxia) culture conditions. Blots were probed with anti-CD9 (top gel), anti-CD63 (middle gel) and anti-CD81 (bottom gel). All exosomes purified using the Kit methodology.
