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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: FEBS Lett. 2017 Mar 19;591(7):979–990. doi: 10.1002/1873-3468.12615

Figure 1. NMR analysis of 15N-Ub in crowded solution.

Figure 1

A) Amide chemical shift perturbations (CSP) obtained from 1H,15N-HSQC spectra of [15N]Ub in the presence of 200 g/L of Ficoll with respect to [15N]Ub in buffer. The CSP data are plotted as a function of residue number. B,C) 15N–spin relaxation rates of [15N]Ub with/without Ficoll. Shown are 15N-R1 (B) and 15N-R2 (C) values as a function of residue number, obtained with (red) and without (black) 200 g/L Ficoll at 25 °C.