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. Author manuscript; available in PMC: 2018 Apr 1.

Published in final edited form as: FEBS Lett. 2017 Mar 19;591(7):979–990. doi: 10.1002/1873-3468.12615

A) In red, ¹H_N-R_2p rates of a representative group of Ub residues, that increase linearly by addition of UBA2 to Ub. The dashed line corresponds to the least-squares linear fit over three experimental points. As control, ¹H_N-R_2p rates measured upon addition of GB1 to Ub, are reported in green. Rates are plotted versus the protein/Ub molar ratio. B) ¹H_N-R_2p rates of representative Ub residues, whose value at 1:0.5 was above the linear trace connecting points 1:0 and 1:1, are shown in red. The dashed line corresponds to the least-squares linear fit over three experimental points. ¹H_N-R_2p rates measured upon addition of GB1 to Ub are shown in green. Rates are plotted versus the protein/Ub molar ratio.

A) In red, ¹H_N-R_2p rates of a representative group of Ub residues, that increase linearly by addition of UBA2 to Ub. The dashed line corresponds to the least-squares linear fit over three experimental points. As control, ¹H_N-R_2p rates measured upon addition of GB1 to Ub, are reported in green. Rates are plotted versus the protein/Ub molar ratio. B) ¹H_N-R_2p rates of representative Ub residues, whose value at 1:0.5 was above the linear trace connecting points 1:0 and 1:1, are shown in red. The dashed line corresponds to the least-squares linear fit over three experimental points. ¹H_N-R_2p rates measured upon addition of GB1 to Ub are shown in green. Rates are plotted versus the protein/Ub molar ratio.