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. 2017 Apr 26;8:607. doi: 10.3389/fpls.2017.00607

FIGURE 4.

FIGURE 4

H3K27me3 abundance on selected genes from C. merolae. Chromatin immunoprecipitation was performed using H3K27me3 antibody (#C15410195, Diagenode) and IgG (#C15410206, Diagenode) for negative control, followed by RT-PCR analysis on a putative H3K27me3 target (CmMADS) and housekeeping genes as the putative non-H3K27me3 targets (Cm60S, CmEIF4A). Selected genes relate to following locus IDs from C. merolae genome project: CmMADS – CMA095C, Cm60S – CMS262C, CmEIF4A – CMK028C. Error bars correspond to the standard deviation from three biological replicates. Significance was calculated using Student’s t-test for the comparison between CmMADS and either, Cm60S, or CmEIF4A. A triple asterisk indicates the significance level of p < 0.001.