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. 2017 Apr 21;9(1):1317578. doi: 10.1080/20002297.2017.1317578

Figure 2.

Figure 2.

Sirtuin 1 (SIRT1) downregulates P.e LPS–induced MMP-13 expression in MC3T3-E1 cells. MC3T3-E1 cells were treated with 20 μg/mL of P.e LPS for the indicated time periods. (a) SIRT1 expression was determined by Western blot. (b) MC3T3-E1 cells were transiently transfected with SIRT1 siRNA or negative control (NC) siRNA for 48 h, and then the mRNA expression of SIRT1 was determined by real-time PCR. (c) The cells were stimulated with 20 μg/mL of P.e LPS or vehicle for 24 h after transfecting with SIRT1 siRNA or NC siRNA for 48 h, and then MMP-13 mRNA expression was determined by real-time PCR. MC3T3-E1 cells were left untreated (control), stimulated with 20 μg/mL of P.e LPS for 24 h only (P.e LPS), pretreated with 50 μM of resveratrol for 1 h followed by P.e LPS stimulation (RES + P.e LPS), or pretreated with 10 μM of EX-527 for 1 h followed by P.e LPS stimulation (EX + P.e LPS). MMP-13 expression was determined by (d) real-time PCR and (e) Western blot, and MMP-13 release was determined by (f) ELISA. *p < 0.05.