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. 2017 Apr 21;9(1):1317578. doi: 10.1080/20002297.2017.1317578

Figure 3.

Figure 3.

SIRT1 downregulates NF-κB p65 acetylation and NF-κB transcriptional activity induced by P.e LPS in MC3T3-E1 cells. (a) MC3T3-E1 cells were left untreated (control), stimulated by 20 μg/mL of P.e LPS for 24 h only (P.e LPS), or pretreated with 50 μM of resveratrol (RES + P.e LPS) or 10 μM of EX-527 (EX+P.e LPS) for 1 h before P.e LPS stimulation. Acetyl-p65 expressions were determined by Western blot. (b) MC3T3-E1 cells were transfected with NF-κB-Luc or pGL3 (empty vector). After 24 h, the cells were treated with vehicle only (control), pretreated with vehicle for 1 h, and then stimulated by 20 μg/mL of P.e LPS for 30 min (P.e LPS), or pretreated with 50 μM of resveratrol (RES +  P.e LPS) or 10 μM of EX-527 (EX +  P.e LPS) for 1 h before P.e LPS stimulation. Cells were lysed with passive lysis buffer, and the luciferase activity was measured. The results were normalized with pRL-TK activities. Each column represents the mean ± standard deviation. *p < 0.05.